FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah7797c-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah7797c-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5590755
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC59640.10667611082939603TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA41100.021.2760681
CGTATGC29500.020.93062444-45
TATGCCG28950.020.83617646-47
GAGCACA43650.020.674929
CACACGT36400.020.22521412-13
CGTCTGA36800.020.13426216-17
CACGTCT37050.019.99840414-15
TGCCGTC28150.019.57232548-49
AGTCACC37250.019.44475628-29
AGAGCAC47050.019.3831278
AAGGGGG26050.018.32551272-73
TCGGAAG50600.018.0308233
CCAGTCA40450.018.02390126-27
AGCACAC41800.017.61262510-11
CTCGTAT32750.016.89574842-43
ATCTCGT33650.016.30270840-41
CTCCAGT46400.016.07091724-25
CCGTCTT34650.016.03782350-51
ATCGGAA58350.015.7995652
AGATCGG36450.015.1243191
Produced by FastQC (version 0.11.2)