FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah7797b-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah7797b-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9774097
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC140960.14421792621865734TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC43150.019.0977448-49
GCCGTCT39050.018.48782748-49
CGTATGC47700.017.32583644-45
TATGCCG50150.016.62147146-47
AGGGGGG30500.016.58571472-73
TCACGCC51550.015.9857930-31
AGTCACG53600.015.6845428-29
GTCACGC49800.014.6877328-29
CTCGTAT56800.014.63366142-43
ATGCCGT50650.014.4881346-47
GTATGCC51600.014.40548844-45
TATCTCG52450.013.76449638-39
AAGGGGG37850.013.30222872-73
CCGTCTT61200.013.27113250-51
CACGCCA55900.013.12743930-31
ATCTCGT63700.012.97397640-41
TCGTATG57750.012.95364142-43
ACGCCAA78600.011.11875932-33
TCTCGTA67050.011.05068340-41
CCAGTCA77850.010.98192126-27
Produced by FastQC (version 0.11.2)