FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah7797a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah7797a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6284161
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32550.020.64738348-49
TATGCCG34650.020.49262246-47
CGTATGC35600.019.81235144-45
AAGGGGG28600.017.35558172-73
CCGTCTT41850.015.718650-51
CTCGTAT34650.015.35233242-43
AGTCACA54250.014.83996828-29
ACACAGT53850.014.81789832-33
CCAGTCA54450.014.698112526-27
TCACACA57750.014.06394430-31
AGTGATC39400.013.86312636-37
ATCTCGT38650.012.71892540-41
CGTCTGA64600.012.46225816-17
CTCCAGT67000.012.33485424-25
CACGTCT65850.012.26175714-15
AGGGGGG25350.012.08566972-73
TGCTTGA60900.011.620658-59
ACAGTGA66350.011.59672534-35
GCCGTCT29950.011.57672248-49
CACACGT68800.011.49442112-13
Produced by FastQC (version 0.11.2)