FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah7602red1-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah7602red1-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610836
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT19300.010.95112314-15
CACACGT18500.010.91121712-13
TGCCGTC17800.010.80664850-51
GAGCACA26300.010.4743789
GATCGGA24650.010.4103441
TATGCCG19650.010.15179248-49
CGTCTGA20100.09.92451216-17
AGAGCAC30750.09.42194758
AGCACAC22150.09.00599210-11
GCACACG15300.09.00248710-11
CGTATGC21900.08.89192346-47
TCGGAAG32750.08.8500313
CCAGTCA22700.08.57855226-27
ATCGGAA39950.08.2069512
GCCGTCT16550.08.179049550-51
CTCGTAT23350.08.1363444-45
AGGGGGG10900.08.06244974-75
ACACGTC17550.07.983638312-13
ACGTCTG17100.07.91598114-15
AGTCACA26250.07.87074628-29
Produced by FastQC (version 0.11.2)