FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah7602i-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah7602i-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8192089
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT255190.3115078461671986TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA98050.028.677449
AGTCACG59700.028.12410428-29
ACGAGTG59600.027.89236832-33
TGCCGTC58550.027.82455350-51
TCACGAG63300.026.5246330-31
AGAGCAC107000.026.456768
TATGCCG64100.025.60064948-49
CGTATGC65000.025.57484446-47
CTCGTAT63650.025.07258844-45
CACGAGT56350.024.9916230-31
AGGGGGG41000.024.50352574-75
GCCGTCT57400.023.91370850-51
GATCGGA116150.023.8943861
CGAGTGG60100.023.5112732-33
GAGTGGA73150.023.08268234-35
GTATGCC60550.023.06170846-47
GTCACGA61350.022.99352328-29
TCGGAAG124450.022.9857543
ATCTCGT70400.022.73607642-43
TATCTCG59600.022.67223440-41
Produced by FastQC (version 0.11.2)