FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah6978i-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah6978i-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5949091
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG158830.2669819641353612TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37950.028.28462648-49
AGAGCAC72300.027.9197629
TATGCCG41600.026.03126546-47
CGTATGC42950.025.6553944-45
GCCGTCT37600.025.57943350-51
ACTGACC43600.025.32737732-33
GATCGGA81750.024.87732
AGATCGG83750.024.3415891
GTATGCC40950.024.29874846-47
CTCGTAT42300.023.74782242-43
TCGGAAG86350.023.6615094
CCGTCTT46500.022.87962250-51
CCAGTCA48800.022.82359726-27
ATGCCGT42950.022.6143448-49
CGGAAGA92200.021.954075
ATCGGAA95800.021.4762633
CGTCTGA52750.021.20439316-17
TCCAGTC48500.020.90822826-27
CAGTCAC48450.020.88061128-29
TCGTATG46700.020.74756244-45
Produced by FastQC (version 0.11.2)