FastQCFastQC Report
Thu 31 May 2018
H2KT5AFXY_n01_ah6978a-0518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KT5AFXY_n01_ah6978a-0518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761346
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC30050.016.3585648-49
CGTATGC38500.013.5083644-45
TATGCCG39750.013.143311546-47
AAGGGGG29700.012.95504272-73
CTCGTAT37750.012.64440342-43
CGATGTA49800.012.06461934-35
CCGTCTT40950.012.00435550-51
ACCGATG53300.011.89615232-33
ATCTCGT42550.011.664494540-41
TCACCGA58250.010.68146930-31
AGTCACC59550.010.60780528-29
AGGGGGG23750.010.50055874-75
GTATCTC52550.010.03231338-39
CCAGTCA63100.010.01101126-27
ATGTATC60200.09.98036636-37
GCCGTCT27600.09.20655450-51
GTATGCC33350.08.33123244-45
CTCCAGT77200.08.30559124-25
TGCTTGA62250.08.24027358-59
CGTCTGA78200.07.956348416-17
Produced by FastQC (version 0.11.2)