FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3x9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3x9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366726
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6660.18160697632564912No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6090.16606403691039084No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6090.16606403691039084No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5750.15679280989076314No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4950.13497815807987434No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4330.11807180292643553No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4010.10934594220208002No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3910.10661911072571892No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3730.10171081406826896No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3690.10062008147772451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA6750.064.478211
TTCCGAT6900.064.092822
CCGATCT6950.062.588574
CGATCTG4450.053.7628825
CGATCTA1903.6379788E-1241.9727755
GCACTAG700.001053046641.4502831
TCCGATC10850.040.09133
TATAGTA951.0277488E-438.1570662
CCTACTC2350.037.0204773
CCGTATA800.002035152236.2492182
GTGCATA1051.8457398E-434.54191
ACAGTGC850.002740023234.116918
GTGCTCT4400.032.9718171
TCTTCCG4050.032.2215275
CTAGGGG950.004723934530.5256543
CTACTCA3151.8189894E-1229.9199874
GGGCGAC1555.491874E-528.0639087
CCCATTA1050.00770300327.618452
GTCATAT2654.5129127E-927.372831
CATATGC3255.0931703E-1126.768653