FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3x8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3x8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221387
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3960.17887229150763143No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3940.17796889609597671No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3710.16757984886194763No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3560.16080438327453736No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3290.14860854521719885No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2740.12376517139669448No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2640.11924819433842095No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2490.11247272875101066No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2460.11111763563352861No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA2390.10795575169273715No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2380.10750405398690979No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2300.10389047234029099No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2250.10163198381115424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT4400.075.8111654
CTTCCGA4500.072.531421
CGATCTG2350.070.972155
TTCCGAT5250.063.5369762
ATAATAC553.2102334E-452.7381973
GTATGTA858.8664456E-751.1986431
CGATCTA1402.6921043E-1046.6168025
TAGGACA803.7206184E-545.3218884
TCCGATC7400.045.076913
GTACCTA657.308543E-444.6347161
GGTGCGG657.3248346E-444.614558
TATACAG855.3214586E-542.65595
GTATAAT907.444504E-540.2952271
TAGGACC750.001479381538.674684
TCTAGGA800.00203112936.257512
AGTATAC1001.3862608E-436.257515
TACACTG1707.76181E-834.1247185
TTTATAC1102.4271008E-432.9613763
GTATAAG900.003614499232.2361831
ATCTGGC1352.1349946E-532.221627
Produced by FastQC (version 0.11.2)