FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3x5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3x5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412812
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7390.17901611387265876No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6020.14582909411548112No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5610.1358972122903404No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5270.12766101760607734No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4970.12039378700231583No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4750.11506448455955737No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4590.11118862823755124No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4260.10319467457341357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA7800.066.008531
TTCCGAT8300.061.1510162
CCGATCT8250.060.642754
CTGTCCG553.2170358E-452.7264379
CGATCTG4750.044.267825
CTAGGGG700.001055372641.4329343
TATACCG750.001481609738.670745
TCCGATC13400.037.3360183
TCTTCCG4400.034.605925
CGATCTC4550.033.465065
GTGCTCT5700.033.0776631
TCGCGCG1551.4624293E-632.7414179
GTCGCGC1601.8738192E-631.7182468
TCCCCCC2853.2196112E-1027.9820128
CGATCTA2603.7016434E-927.887555
TCTATAA1306.472468E-427.887552
TATATCG1050.007699337327.6219545
CCCCCCG3005.838956E-1026.582919
ACCCAGC1409.985548E-425.8924477
ACCATAC4001.8189894E-1225.3807451
Produced by FastQC (version 0.11.2)