FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3x3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3x3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185266
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3400.18351991191044228No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3270.17650297410210183No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3200.1727246229745339No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2820.15221357399630803No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2620.14141828506039963No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2430.1311627605712867No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2130.11496982716742413No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2090.11281076938024245No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1930.10417453823151576No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1860.10039618710394785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG2550.073.939875
CCGATCT4050.064.46044
CTTCCGA4000.063.4703451
ACTACGT350.003558425762.1582456
TTCCGAT4300.060.7127082
GGGCGAC1203.594323E-948.3322537
CCCCCCG752.5428097E-548.3322539
ACACATA803.717364E-545.323723145
GTCGCGC1151.4044963E-744.1294488
GGGGCGA1557.3123374E-1042.10726
CGATCTA700.001053126841.438835
ATCTGGG700.00105452741.4276477
ATCTGGC1053.807807E-641.4276477
ACCTATA951.02252714E-438.1776541
TATAGGT1157.0991937E-637.8354533
GGTCGCG1157.112365E-637.825247
TCGCGCG1157.112365E-637.825249
TCCGATC7150.036.5125353
GATCTGG2600.036.258986
ACTCCCC1051.8452178E-434.532366
Produced by FastQC (version 0.11.2)