FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3x2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3x2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657602
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA39650.064.586811
CCGATCT40350.062.5340844
TCCGATC41700.061.3789833
TTCCGAT42150.060.7283132
CGATCTG21650.056.5991445
CGATCTA8600.041.3122335
GATCTGG12100.038.950146
CGATCTC15450.036.6055685
TCTAACG1001.3901206E-436.2535933
GATCTAG3200.036.2535936
GATCTCA4850.031.3948636
GTGTTAA5400.030.8991371
CGATCTT12600.030.4990565
TAGAACG1453.4862365E-530.0029744
GTGCTCT22650.029.7870181
GATCTGA9150.029.3198476
TCTTCCG23300.029.2518275
CCCATAC1000.006054253329.018321
TATACCG1306.476815E-427.8873795
GTATATA1855.7836914E-627.4497621
Produced by FastQC (version 0.11.2)