FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3x12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3x12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158306
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3660.2311978067792756No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3260.2059302869126881No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3070.19392821497605903No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3060.19329652697939434No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2680.1692923831061362No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2520.1591853751595012No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2380.15034174320619562No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2070.1307594153095903No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2040.12886435131959623No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2040.12886435131959623No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2020.12760097532626685No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2020.12760097532626685No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2000.12633759933293748No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA1950.12317915934961403No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT300.001932740272.499857
CCGATCT3050.064.180194
CTTCCGA2950.063.9183541
TTCCGAT3450.056.7390062
GGGCGAC1051.0986696E-955.2379767
AGCCTAT400.006041569654.374886
CGATCTG1500.053.166555
GTTATTA450.00961234548.34851
GCTGGCC450.00962435548.333225145
CCACGGG450.00962435548.3332252
GTACTTA657.306507E-444.6293831
TGGGGCG855.3211334E-542.6469655
TACAGTA700.00105395741.4284822
ACCATGC907.45399E-540.2776878
TATAAGA907.45399E-540.2776872
CTCCCCC1303.6722122E-739.0383767
ACCATAC1604.5354682E-836.2613751
TTTGGGG800.002031471336.2499243
TCCGATC5550.035.2701953
CCCCCCG1458.6372165E-734.999929
Produced by FastQC (version 0.11.2)