FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3x10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3x10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515269
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA9740.18902747885085266No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8970.17408382805874215No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8220.15952832404045267No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7900.15331797565931582No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7260.14089727889704212No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6900.1339106369682632No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA6290.12207216036672107No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6170.11974327972379475No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6150.11935513294997371No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5440.10557592247932633No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA5330.10344111522331055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA8600.069.969481
CCGATCT8800.067.555414
TTCCGAT9450.065.210292
CGATCTG6000.057.9987955
GTCGCGC2350.040.105558
TCGCGCG2400.039.270029
TCCGATC16400.038.01753
AGGGGGC800.002035907736.249255
TCCCCCC3200.036.249258
TATACAG2600.036.249255
ATAATAC2404.3655746E-1133.2284773
GGTCGCG2850.033.0694927
AGGTCGC2850.033.0694926
GGGCGAC2455.4569682E-1132.5503467
GTGCTCT5500.031.6357081
CCCCCCG3450.031.5210849
CATAATA1851.6412923E-731.35072
TACAGGG1453.4876393E-529.9993782
GTCTAGA1753.770554E-628.99941
CGATCTA2303.4815457E-828.3689775
Produced by FastQC (version 0.11.2)