FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294450
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5940.20173204279164544No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4970.16878926812701647No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4640.1575819324163695No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4270.14501613177109865No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4230.1436576668364748No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4120.13992188826625912No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3520.119544914246901No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3470.11784683307862116No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3410.11580913567668534No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3050.10358295126507047No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2970.10086602139582272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA5000.068.18131
TTCCGAT5250.064.912512
CGATCTG3600.058.4097565
CCGATCT6000.056.798454
TAGGGTG553.2133708E-452.7335745
GTATAAG1004.6711648E-850.773311
TTAATAC604.93645E-448.3391083
ATATTAG1053.8028775E-641.44761
TCTTCCG3150.041.433525
TCCGATC8850.039.3267363
TCTATAC951.026402E-438.1624533
GGCGACC1854.1636667E-935.2684948
TAATAGT850.002737255534.1217236
CCAGGGG850.002737255534.1217233
TATTAGG850.002737255534.1217232
GGGCGAC1501.1307329E-633.831637
AATACTG1551.4589968E-632.745855
GTGCTCT4450.032.5992361
GTATATA900.003615964232.2370221
CCTACTC2051.1315933E-831.8330733
Produced by FastQC (version 0.11.2)