FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214610
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4390.20455710358324403No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3940.18358883556218256No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3680.17147383626112483No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3620.16867806719165No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3240.15097152975164252No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3160.14724383765900936No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2860.13326499231163505No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2840.13233306928847677No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2520.11742230091794417No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2190.10204557103583245No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2180.10157960952425331No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2160.10064768650109501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA3900.070.657371
CCGATCT4050.069.8147054
TTCCGAT4500.061.2221262
CGATCTG2600.044.6153145
TTATATA855.3274773E-542.646994
TATAGTC700.001054793741.4285053
ATATGCC750.001480800238.6666033
TAGGCGG850.002737233634.117594
TCCGATC8400.033.660663
TCCCCCC1102.4298337E-432.954498
TAGAGGT900.003621961732.222174
GTGCTCT3000.031.4239371
CCTAGAG950.004719150730.5262682
TGCGACC950.004719150730.5262685
TTGCGAC950.004719150730.5262684
CAGTGGG950.004719150730.5262689
ATACAGC950.004719150730.5262686
ATGTAAG1000.006057118529.0067121
GGTCGCG1000.006064021528.9999567
GGGCGAC1000.006064021528.9999567
Produced by FastQC (version 0.11.2)