FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126561
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2570.20306413508110713No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2430.19200227558252542No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2360.18647134583323458No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2080.16434762683607115No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1940.15328576733748944No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1930.15249563451616216No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1630.1287916498763442No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA1470.11614952473510798No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1460.11535939191378072No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1390.10982846216448985No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1360.10745806370050805No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1350.10666793087918079No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1340.10587779805785351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT2700.083.240524
CTTCCGA2750.081.7593461
TTCCGAT2850.078.8594362
GACCGTA300.001931844572.49987
TGGGGAC300.001931844572.49985
TCTTCCG902.7830538E-1064.444275
CGATCTG2050.060.1217845
CGATCTC1353.6379788E-1253.703565
TCTACAC553.208295E-452.727133
CCCCCCG701.6875354E-551.7855769
TATAGCA701.6875354E-551.7855764
TCCGATC4400.051.0794073
GCGACCC1301.2914825E-1050.192179
CCCTTAT450.00960493148.3523031
GGTGATA450.00960493148.3523031
ATATAGC604.928681E-448.3332023
TCCCCCC752.537907E-548.33328
CTGGGGA450.00961994748.33324
GGGCGAC1255.135007E-946.399877
GTATATA657.298093E-444.6328961
Produced by FastQC (version 0.11.2)