FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410982
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG4140.10073433873016337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA26250.049.1668551
TTCCGAT26900.048.2483522
CCGATCT27800.046.164724
TCCGATC28800.045.0653043
CGATCTG13200.045.0424165
TATGTCG800.002034215736.253655
GTATAGC1102.4322269E-432.9578631
TATACCG1102.4322269E-432.9578635
CGATCTA5450.031.9298215
ACACATA2601.10958354E-1030.676165145
GTATATG950.004721764530.5293881
GTCCTAT1255.1455887E-429.002921
GGATATG2252.8057912E-829.002921
GTGTTAA4750.029.0029181
ATCTGGC4500.028.9993927
CGATCTC13050.028.3361875
GTTAAGA4900.028.1150763
GGATAGG1306.47259E-427.8874231
TGTACTG1358.0698205E-426.8545555
GTGCTCT18850.026.5411341
Produced by FastQC (version 0.11.2)