FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142765
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3000.210135537421637No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2730.19122333905368963No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2580.18071656218260776No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2410.16880888172871503No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2330.16320526739747138No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2290.16040346023184954No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1860.13028403320141493No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA1850.12958358141000945No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1830.12818267782719855No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1660.11627499737330578No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA1650.11557454558190033No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1590.11137183483346759No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1530.10716912408503484No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1480.10366686512800756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG259.3701534E-487.01
CTTCCGA3050.061.803281
GTCATAA502.0043855E-458.01
CCAGCGC502.0043855E-458.09
TTCCGAT3350.056.2686542
CCGATCT3450.054.637684
TCTTCCG1800.052.361115
CGATCTG1457.2759576E-1250.05
CCCTAAG450.00962234748.3333321
CTAACTG450.00962234748.3333325
GACGCTC657.315386E-444.6153878
CGATCTA1201.9575964E-742.2916685
CAGTATG700.001053597441.428575
GTGCTCT2800.038.8392831
AATCCAG750.001479125638.6666646
AGACGCT750.001479125638.6666647
AAACACG1001.3854833E-436.257
CTATGGG850.002734155934.1176454
TAAGGTG1102.4257546E-432.9545445
TCCGATC5850.032.2222253
Produced by FastQC (version 0.11.2)