FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365560
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT5950.075.544874
TTCCGAT5950.075.544872
CTTCCGA6000.074.915331
CGATCTA809.822543E-1172.49875
CGATCTG3400.061.8371285
TCCGATC7550.059.535363
CGATCTT1851.8189894E-1243.1073345
TCTTCCG3550.034.7176865
TATAGTG1751.0063741E-733.142265
ATAGTGC900.003625562232.2216456
CGATCTC3750.030.932785
GATCTAA1504.391706E-528.9994811
TCGCGCG1255.1480625E-428.9994819
TAACGCC1358.073698E-426.8513724
ACACCGT1100.00966455826.3631636
CTATAAG2507.8036464E-826.0995332
CGTCATG2808.1490725E-925.895935145
GTCGCGC1409.983476E-425.8923918
GTATATA3851.6370905E-1124.480081
CGAGACA1500.001491430624.16954145
Produced by FastQC (version 0.11.2)