FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155745
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2800.17978105236123149No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2630.16886577418215673No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2340.15024559375902918No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2320.14896144338502038No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2080.13355163889691482No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1790.11493145847378727No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1730.11107900735176088No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA1700.1091527817907477No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1640.1053003306687213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA2950.068.813561
CGATCTG1950.063.2051245
CCGATCT3350.062.7611924
TTCCGAT3450.058.840582
GTAGGTG400.00604133454.3749968
TCTTCCG1750.049.7142835
GGGCGAC1056.856135E-848.3333367
TCCCCCC752.5405881E-548.3333328
TCCGATC5000.042.053
ACCAGCG700.001053892441.428578
CCCCCCG907.4533295E-540.2777759
TACACAG907.4533295E-540.2777755
TAGAACA750.001479538938.6666644
CTCCCCC951.02489736E-438.157897
CCTATAA1752.401066E-937.2857131
GGCGACC1458.6358705E-735.08
GTACGAC850.002734915134.1176453
TACTCTG1301.6458616E-533.461545
GCGACCC1601.8636892E-631.7187489
TACTCCC1601.8636892E-631.7187485