FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3h10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3h10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300320
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5160.17181672882258925No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4920.16382525306339904No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4900.1631592967501332No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4360.14517847629195524No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4230.1408497602557272No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4070.13552210974960044No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3750.12486680873734683No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3310.11021576984549815No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3170.10555407565263719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT5150.066.165054
CTTCCGA5700.061.0526351
TTCCGAT6100.055.8606572
CGATCTG3100.051.4516145
TCCTGGG858.903735E-751.176472
AGGGGAG752.5462843E-548.3333365
GGGCGAC1700.046.9117667
TAGGTGA750.001481731838.6666684
CCCCCCG2201.6370905E-1136.2500049
TCCCCCC2302.5465852E-1134.6739168
CAATAGT850.002738945234.117654
TCCGATC10350.033.6231883
CTCCCCC2603.6379788E-1233.4615367
TATACAG900.003624220432.222225
GGCGACC2950.031.9491548
TCTTCCG3450.031.521745
TCTAATG1402.7403321E-531.071432
GCGACCC3050.030.901649
ACTCCCC2859.094947E-1230.5263186
CGATCTT2901.2732926E-1130.0000025
Produced by FastQC (version 0.11.2)