FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243373
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5760.23667374770414135No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4990.20503506962563636No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4960.20380239385634397No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4840.19887169077917435No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4390.18038155423978833No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4130.16969836423925416No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3900.1602478500080124No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3760.15449536308464784No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3430.14093592962243143No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA3280.1347725507759694No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3250.133539875006677No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3180.13066363154499472No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA2860.11751509000587576No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT2820.1158715223134859No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA2780.11422795462109601No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2740.11258438692870615No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2710.11135171115941374No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2670.10970814346702387No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA2500.10272298077436692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA4800.066.4696351
GGGCGAC1350.064.442167
TTCCGAT5100.061.1252862
CCGATCT5300.060.1865464
CGATCTG3000.057.9979485
GGGTACC553.214718E-452.7254076
GTACTAC701.6904592E-551.7945251
GCGACCC1900.049.6035049
TATACCG450.00963178848.3316235
GGCGACC1900.045.787858
GGGGCGA2400.042.290176
CGATCTA1303.6840356E-739.0370835
GTCTATA750.001479941138.6732441
ACTAGTA800.00203394736.248722
TCTTCCG3000.033.8321345
TCCCCCC1801.285971E-732.221088
GTGCTCT4200.031.0767141
CCTTAAA1402.7388765E-531.0703282
CGATCTC2601.09139364E-1030.6719935
GGTACAT950.00471641730.5315071
Produced by FastQC (version 0.11.2)