FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154649
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3340.21597294518554921No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3330.21532631960116136No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3320.21467969401677348No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2830.18299504038176775No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2500.16165639609696797No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2450.1584232681750286No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2120.13708462389022882No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2050.13255824479951372No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT1920.12415211220247141No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1860.12027235869614417No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1850.11962573311175631No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1780.11509935402104118No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA1750.11315947726787759No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1670.10798647259277461No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA1620.10475334467083525No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGC353.4280733E-582.857154
CTTCCGA3450.077.7536241
CCGATCT3600.076.527784
TTCCGAT3650.073.493152
GGGCGAC955.456968E-1268.6842047
GTATAGA451.1906803E-464.444441
CGATCTG2450.062.1428575
GGCGACC1203.587047E-948.3333328
AGTACAC450.00962384748.3333323
GTAGTAC604.932033E-448.3333321
TAGTACT752.5405037E-548.3333324
TCCGATC5900.047.9237333
GGGGCGA1850.047.027036
GTGCTCT1900.045.789471
TCTTCCG1950.044.6153875
GCGACCC1351.0162694E-842.9629639
CTCTATG951.0248637E-438.157892
ATCCTAG800.00203130336.251
ATAGACC800.00203130336.253
CTTGCGA1001.3860158E-436.259
Produced by FastQC (version 0.11.2)