FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599846
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12030.20055147487855218No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG11880.19805083304714877No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC11510.19188258319635373No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA10960.18271356314787462No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9770.16287513795207437No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8370.13953581419230937No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC7740.1290331185004151No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7180.1196973889965091No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA7100.11836371335309395No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6800.11336242969028718No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG6790.11319572023486028No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6670.11119520676973757No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG6490.10819443657205348No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT6330.1055270852852232No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT6170.10285973399839293No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC6070.10119263944412399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA39000.072.162561
TTCCGAT39550.071.129372
CCGATCT42050.066.5556644
TCCGATC48650.058.1226653
CGATCTG20200.057.788085
CGATCTA9100.056.5692835
TATAGCG400.006048292454.378225
CGATCTC17200.043.4182665
GATCTGA5550.043.110666
CTAGGCC700.001056428241.427579
GATCTAG4400.039.54786
GATCTAC5750.037.8283276
ATCTAAC1951.70985E-1037.178597
ATCTAGG2052.910383E-1035.3649987
TCTGTCC1854.1782187E-935.269428
CGATCTT11100.034.6191675
TTATACG1051.852189E-434.5258564
TGCTCTT22550.034.403372
TCTTCCG22900.034.1941645
AGCCTAT850.002740318234.119675
Produced by FastQC (version 0.11.2)