FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379040
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA23400.054.5638241
TCTAGGC553.2086798E-452.7530982
TTCCGAT24250.052.6443252
CCGATCT24400.050.8276024
CTATACA1600.049.861584
TCCGATC25450.049.5855143
CGATCTA4450.048.893895
CGATCTG10900.045.910915
GGACTGA951.02552025E-438.1715436
ATCTAGG800.00202882136.272541
ATACTTA800.002031452336.2629666
TCTATAC1803.1777745E-936.2629663
CGATCTC8050.035.1367875
TACAGGG850.002733276234.1343572
GATCTGG7050.033.948316
TACACTA1501.1282664E-633.8454365
CTCTATA1551.4562465E-632.757972
TCTCTAT1801.2817873E-732.2422561
TCTACAC2807.2759576E-1231.0825443
GATCTAG1652.3746325E-630.768586
Produced by FastQC (version 0.11.2)