FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185855
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4730.2544994753974873No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4690.2523472599607221No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4150.2232923515643916No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4040.21737375911328724No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3610.1942374431680611No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3400.18293831212504372No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3170.1705630733636437No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3140.1689489117860698No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2960.1592639423206263No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2840.15280729601033063No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA2630.14150816496731322No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2530.13612762637540018No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2460.13236124936106103No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC2410.1296709800651045No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2310.12429044147319146No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2250.12106211831804364No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA2250.12106211831804364No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2200.11837184902208711No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA2160.11621963358532188No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT2100.11299131043017406No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA2060.11083909499340883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT4150.075.1195144
CTTCCGA4200.072.499061
TTCCGAT4400.069.203642
CGATCTA1301.8189894E-1255.7685055
CGATCTG2650.051.9804575
TCTGCGC450.009627379548.3327068
ATAATAG604.9349415E-448.3327063
GTTATAA951.9125673E-645.7888761
TATAATA803.7231403E-545.311912
CCCCCCG1351.0184522E-842.9624069
TCCCCCC1351.0184522E-842.9624068
TATATTG700.001054487541.4280325
ATAACGC700.001054487541.4280329
TCCGATC7850.039.712863
CATAGAC750.001480371738.6661643
GATCTAC1157.112034E-637.8255966
CTCCCCC1553.444802E-837.418877
CGATCTT1752.406523E-937.285235
TCTTCCG2750.036.908615
ACTCCCC1854.143658E-935.2698146
Produced by FastQC (version 0.11.2)