FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261425
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5330.2038825667017309No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5270.20158745338051065No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4930.18858181122692932No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4600.17595868796021805No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4530.17328105575212777No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4000.15300755474801567No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3600.1377067992732141No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3500.1338816104045137No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3320.12699627044085302No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3200.12240604379841255No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3180.12164100602467247No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3100.11858085492971214No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT3030.11590322272162189No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3030.11590322272162189No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2910.11131299607918142No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC2820.10787032609735105No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2720.10404513722865066No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2720.10404513722865066No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA2660.10175002390743043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA5400.071.169141
CCGATCT5800.064.998284
GGGGTTA350.003563931262.1412126
TAGGCGA350.003563931262.1412124
CGATCTG3400.061.8366055
TTCCGAT6250.061.478382
TATACCA502.0077478E-457.998475
TAGAGCG553.2150175E-452.7258835
GTCGCGC1600.049.8424348
TCGCGCG1600.049.8424349
CTAGGCG604.938979E-448.3320583
GTAATAC700.001055345441.4274753
GGTCGCG1953.6379788E-1240.896367
TCCGATC9900.038.812113
ACTAGGG750.001481572738.6656463
TAGATCG951.026968E-438.156895
GGGCGAC1354.95369E-737.5916027
AGGTCGC2252.0008883E-1135.443516
TCTTCCG3500.035.2133565
TATGGCG850.002738653434.116753
Produced by FastQC (version 0.11.2)