FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154596
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3420.2212217651168206No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3210.20763797252192812No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3210.20763797252192812No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2770.17917669279929624No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2510.16235866387228648No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2420.1565370384744754No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2420.1565370384744754No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2300.14877487127739397No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2220.14360009314600636No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1990.12872260601826696No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA1860.1203135915547621No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1840.11901989702191519No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA1690.1093171880255634No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA1640.10608295169344614No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT1640.10608295169344614No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC1610.10414240989417578No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1580.10220186809490543No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG1580.10220186809490543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA3800.078.273991
CCGATCT4150.071.626184
CGATCTG2600.066.922785
TTCCGAT4500.066.055262
TATATAC554.045498E-665.90883
CGATCTA902.0345396E-856.3886345
TATTATG701.6893091E-551.7854842
ATAGGGT450.00962401348.3331154
TCCGATC6350.046.8108143
TAAGGTG1651.3496901E-939.5452775
GGCGTGC750.00147953738.6664967
AGGTGCC1701.8080755E-938.3821837
CAGTGGG850.002734911934.1174969
TCATAAG2404.3655746E-1133.2290152
TCTTCCG2008.8475645E-932.6248555
ATAAGGT2008.8475645E-932.6248554
CATAAGG2051.1254087E-831.8291263
ATCTAAG950.00471517230.5261822
TCCTATC1204.0271398E-430.2277581
CTCATAA2401.54796E-930.2277581
Produced by FastQC (version 0.11.2)