FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3g10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3g10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225896
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5390.23860537592520453No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4670.20673230159011227No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4490.1987640330063392No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4420.1956652618904275No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3980.17618727201898218No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3660.16202146120338565No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3520.15582391897156214No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3450.1527251478556504No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3130.13855933704005383No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2860.12660693416439425No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2660.11775330240464639No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA2510.1111130785848355No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2440.10801430746892374No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2420.10712894429294897No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA2380.1053582179409994No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2320.10270212841307505No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA2280.10093140206112547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA4100.068.9916461
CCGATCT4500.062.859054
TTCCGAT4700.061.7273832
GGGCGAC1300.061.3440937
CAGGGGC651.0882779E-555.7920574
TACTGGG400.006045649354.3731777
TCTTCCG2550.054.041725
GGCGACC1600.049.842088
GCGACCC1700.046.910199
CGATCTG3150.046.0505875
TATACAG951.9088493E-645.808225
GTGCTCT3400.040.5312921
TCAGGGG750.00147797638.68253
TATGACG750.00147797638.68254
TAAACTG1209.504383E-636.264845
TACAGGG1001.384702E-436.264842
TCCGATC8250.035.1659053
TGCTCTT4200.032.8110472
ATCTTAC900.003615076232.2354131
CTTGCGA1352.1354028E-532.221149
Produced by FastQC (version 0.11.2)