FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315481
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6310.20001204509938791No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4920.1559523394435798No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4760.15088071864866665No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4530.14359026375597897No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4130.13091121176869605No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4060.12869237767092156No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3630.11506239678459242No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3440.10903984709063304No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3340.10587008409381231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT6900.061.9920734
TTCCGAT6800.059.705232
CTTCCGA7050.058.6442641
CGATCTG3450.052.5356565
TCCGATC11050.037.397783
TCTTCCG4100.035.3654675
CTATAAT1051.8457988E-434.5398561
CTGTGTA850.002739300534.1172759
TTAGGAG1352.1377446E-532.221873
ACCTAGC900.0036246932.221873
CGATCTA2257.876224E-1032.221875
GTGCTCT4350.031.6813851
CGATCTC4150.031.4454385
CCATAGG950.004711718830.5405041
CTATATA1255.1462726E-428.9996812
ATATACT1753.7641712E-628.9996814
GTTCGTA1358.048503E-426.8643321
TAATCTA1409.980022E-425.8925764
TTACATA1701.0291781E-425.5879574
CTTGCGA2301.0992171E-625.2171179
Produced by FastQC (version 0.11.2)