FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749138
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC13370.17847179024425408No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG12290.16405522080043997No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG11550.15417720099634513No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT9260.12360873430529487No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8060.10759032381216813No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC7580.10118295961491743No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT7510.1002485523361517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT43300.061.6231272
CCGATCT44150.059.7798044
CTTCCGA45450.059.2103771
CGATCTG21900.056.9466445
TCCGATC47800.056.125163
TCTAGGC907.475994E-540.2820243
GATCTGG10550.039.8620226
CGATCTA9850.035.3336755
CGATCTT14050.033.028395
GGCCCTA2653.6379788E-1232.8292667
GTGCTCT23500.032.410011
GATCTAG3550.030.6370326
TGCTCTT25550.030.0814482
ATCTGAC4850.029.8960697
ATCTGCC5350.029.8122467
GATCTGA9150.028.5275976
TATAAGC4650.028.0674743
TAGACGT3453.6379788E-1227.321724
CTATAAG5050.027.2801042
GGGCCCT3204.1836756E-1127.1903676