FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256111
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6280.24520618013283302No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5700.22255974948362234No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5250.20498924294544163No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4900.1913232934157455No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4640.18117144519368555No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4640.18117144519368555No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4250.1659436728605956No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4190.16360093865550485No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3780.14759225492071798No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3670.1432972422113849No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3170.12377445716896189No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3100.12104126726302268No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3100.12104126726302268No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA3040.11869853305793192No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG3010.11752716595538654No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2940.11479397604944731No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC2830.11049896334011425No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA2780.10854668483587195No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT2730.10659440633162966No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT2720.1062039506307812No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2660.10386121642569042No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG2660.10386121642569042No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA2600.10151848222059966No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC2570.10034711511805429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT4850.076.234962
CCGATCT5000.072.497954
CTTCCGA5250.070.4540941
TGGCCCT350.003563874262.14115
CGATCTA1300.061.3444185
GTATTAT651.0889955E-555.7894361
CGATCTG2650.054.7154355
CCTTATA450.00963208948.3319662
TTAATAC657.3273457E-444.614123
TCTTCCG2550.042.6458555
CAATGCG750.001481530438.6655739
TCCGATC9900.037.3474273
GTGCTCT3150.036.838741
GTATATA1001.385604E-436.2631381
GTCGCGC1458.666775E-734.9990128
TCGCGCG1458.666775E-734.9990129
TTAACAC1251.2616678E-534.7990153
TATAGGT1601.8703267E-631.7178543
TAATCGT1153.1561073E-431.520854
GGGAGAC1402.7392987E-531.070556
Produced by FastQC (version 0.11.2)