FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201460
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4010.19904695721234986No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4000.19855058076044874No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3680.18266653429961283No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3570.1772063933287005No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2970.1474238062146332No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2600.12905787749429165No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2400.11913034845626924No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2340.1161520897448625No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2250.11168470167775241No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2200.10920281941824679No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2180.10821006651444455No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2080.10324630199543333No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA2020.1002680432840266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA4150.073.409691
CCGATCT4250.071.646824
TTCCGAT4700.064.787022
CCTAGGG400.00604398354.3748172
CAGGGGA553.2128787E-452.7270939
CGATCTG2100.051.7855385
TCCGATC7350.041.428433
CGATCTA907.4612995E-540.277645
TTAGTGT800.002032797636.2498784
CTACTGG850.002730203834.1344761
CCTACAC1301.6482963E-533.4614223
CGGGCCT1352.1338232E-532.22211145
TTAACAC900.003621470432.222113
CCCATGC3250.031.2306638
CCGCTAA950.00470706230.5413721
CTAAACA950.00471851330.5262134
TCCTACA1702.9960902E-629.852842
TACACCT1702.9960902E-629.852845
CCATGCT3750.028.99999
TCCCATG3750.028.99997
Produced by FastQC (version 0.11.2)