FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130083
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3160.242921826833637No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3050.23446568729195974No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2990.229853247541954No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC2730.2098660086252623No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT2400.1844975900002306No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2270.1745039705418848No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2210.16989153079187905No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA1900.1460605920835159No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1800.13837319250017296No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA1780.13683571258350438No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1750.1345294927085015No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT1660.12761083308349283No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1650.12684209312515854No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA1620.12453587325015567No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC1600.12299839333348708No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA1570.12069217345848421No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1470.11300477387514125No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT1460.11223603391680698No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT1440.11069855400013837No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG1320.10147367450012684No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG1320.10147367450012684No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC1320.10147367450012684No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA1310.10070493454179255No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1310.10070493454179255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGC353.426246E-582.856054
TACTATA300.001932045972.499042
CTTCCGA2400.069.478251
GGGCGAC754.811227E-967.665777
CCGATCT2600.066.922194
CGATCTA702.3188477E-762.1420365
CGATCTG1500.057.999235
TTCCGAT2950.056.5246732
TCTTCCG1600.054.374285
CTACATG400.00603941954.374283
ACCGATC553.2087928E-452.7265788
TTGCGAC553.2087928E-452.7265784
CCGATCA901.3147546E-648.332699
AGTATTG450.00962093948.332693
CTTGCGA752.5384557E-548.332693
GGCGACC1109.870928E-846.1357548
CAAGGCG855.3162184E-542.6464969
TGCGACC700.001053319341.4280245
TATACAT700.001053319341.4280244
GCGACCC1252.694942E-740.599469
Produced by FastQC (version 0.11.2)