FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613084
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT38600.065.931172
CTTCCGA38900.065.630511
CCGATCT38800.064.283234
CGATCTG19250.062.1475755
TCCGATC41150.061.8455243
CGATCTA7600.047.7009935
GATCTGA7550.039.373856
CGATCTC16800.036.6843345
GATCTCA5250.035.907496
CTATACT2103.765308E-1034.5264324
GATCTGG11550.034.526436
GCTATAC1601.8744377E-631.7211593
TATACTG3050.030.9039845
CGATCTT11100.030.7005315
CCTAATG950.00472412830.5286352
CTAGGGT950.00472412830.5286353
TGCTATA1453.4864068E-530.0022772
GTGCTCT21550.029.617481
TCTTCCG23300.028.0064185
ATCTGGG5200.027.8844597
Produced by FastQC (version 0.11.2)