FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507176
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA34000.057.3859331
CCGATCT34100.056.3501474
TTCCGAT34650.056.0835042
TCCGATC35500.054.9449163
CGATCTG16300.050.2683265
GATCTCA4750.038.1636546
CGATCTC13150.038.047575
CGATCTT10500.036.600765
CGATCTA6550.036.532235
TCTACAC3350.034.632093
GTGCTCT21100.032.6567731
TGCTCTT21350.032.2648242
TTAGACG900.003623878132.2270853
TCTTCCG22600.031.4427995
GATCTGC10500.030.3855386
GCTCTTC23700.029.0655673
GTGTAGG1255.1368464E-429.0129621
TATACCG1255.1457516E-429.004385
TCTGTCC1804.6956575E-628.193148
TATAGGT4150.027.9560283
Produced by FastQC (version 0.11.2)