FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549028
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC28300.5154564065949278No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG18510.33714127512622305No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG18330.3338627538121917No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17610.3207486685560664No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT16190.29488477818981906No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14140.25754606322446216No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT13710.24971404008538728No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG13630.24825691950137335No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA13490.24570695847934898No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG13470.24534267833334547No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG12760.23241073315022187No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12480.22731081110617307No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT12080.2200252081861034No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT12030.21911450782109476No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11750.21401458577704596No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11480.20909680380599896No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG11310.20600042256496937No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC11070.20162906081292756No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT10760.19598271854987362No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC10580.19270419723584226No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG10410.18960781599481263No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC9840.17922583183371338No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA9750.17758657117669774No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC9710.17685801088469078No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG9370.17066524840263156No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA9300.16939026789161937No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG9250.16847956752661067No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT9180.16720458701559848No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT9120.16611174657758804No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA8970.1633796454825619No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8930.16265108519055493No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA8770.15973684402252708No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8670.15791544329250967No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC8540.15554762234348704No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT8490.15463692197847834No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8180.14899057971542437No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8170.1488084396424226No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC8100.14753345913141042No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC7940.14461921796338256No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT7880.1435263775253721No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA7750.1411585565763495No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT7740.14097641650334775No Hit
CCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCG7690.140065716138339No Hit
ATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGT7450.13569435438629723No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG7350.13387295365627983No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG7310.13314439336427286No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA7280.13259797314526764No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC7270.1324158330722659No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC7200.1311408525612537No Hit
TAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAG7090.12913731175823456No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7030.12804447132022412No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG7020.12786233124722238No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT7010.1276801911742206No Hit
CTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAA6970.12695163088221365No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGA6840.12458380993319103No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG6840.12458380993319103No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTT6810.12403738971418579No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC6580.11984816803514574No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG6510.11857318752413355No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC6320.11511252613710048No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC6200.11292684526107957No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA6190.11274470518807785No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC6060.1103768842390552No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACT6010.1094661838740465No Hit
ATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAA5950.10837334343603605No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC5930.10800906329003256No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC5930.10800906329003256No Hit
GTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGA5920.10782692321703083No Hit
CCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT5910.10764478314402909No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC5910.10764478314402909No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG5860.10673408277902037No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT5780.10527696219500644No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA5760.10491268204900298No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5720.104184121756996No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT5610.10218058095397685No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA5590.10181630080797335No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA5590.10181630080797335No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC5550.10108774051596639No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG5520.10054132029696117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA28050.089.985521
TTCCGAT28500.088.794932
CCGATCT29400.085.3290254
TAGTATA406.655228E-572.511612
GATCTAC2800.072.505016
CGATCTC9350.069.0154655
CGATCTA5700.068.688965
CGATCTG16950.066.302515
TCCGATC39800.063.7606853
CGATCTT5850.060.730695
ATCTGAG1650.057.1199537
GATCTCT2950.056.529336
GATCTGA4800.052.868246
TGCTCTT10750.052.6130752
GTGCTCT11100.052.2749061
ATCTGAC2550.051.1753437
TCTTCCG11500.048.546835
GTCCTTA604.932562E-448.354291
CAGTATG1751.8189894E-1245.578728145
GATCTCA3050.045.1670576
Produced by FastQC (version 0.11.2)