FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733557
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC15370.20952700335488583No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG10660.14531931397287465No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG10600.1445013816240592No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10300.14041171987998208No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA9620.13114181992674054No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT9390.1280064125896147No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8830.12037237733400404No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG8650.11791858028755775No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG8380.11423788471788832No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT8360.1139652406016165No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG8360.1139652406016165No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8230.11219305384584974No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8020.10933029062499575No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT7910.10783074798550078No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT7680.10469534064837496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA40600.064.115821
TTCCGAT41450.062.4426422
CCGATCT41050.062.3446464
TCCGATC46900.055.34113
CGATCTG21450.052.7272225
CGATCTC16950.043.2005465
CTAGGGG1851.8189894E-1243.108073
CGATCTA8000.042.593715
ATCTGAG3500.035.2142527
CGATCTT11550.034.523785
TCTAGGC1751.00857505E-733.1428263
GATCTGG11600.032.499976
TCTTCCG23650.031.8815785
GATCTGA8350.031.2574586
TAAGCTA950.004726505430.5262855
GTGCTCT24500.030.4837191
GATCTAG4050.030.432076
TTATACT3350.030.298484
CTAGGCT1204.0570242E-430.2083054
TCTAAGC1703.0102965E-629.8529113
Produced by FastQC (version 0.11.2)