FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241735
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4220.17457132810722484No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4210.17415765197426936No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4080.16877986224584773No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3900.16133369185264856No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3510.14520032266738372No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3080.1274122489502968No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2910.12037975469005316No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2730.112933584296854No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2680.11086520363207644No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2610.10796947070138788No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA2510.1038327093718328No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2460.10176432870705523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT4450.057.022314
TTCCGAT4450.055.39312
CTTCCGA4800.051.3752751
CGATCTG2550.048.33325
GGGCGAC1201.9651998E-742.2915467
CCTACAC1401.4069883E-841.428453
ATCTGCC907.465713E-540.277667
GCGACCC1303.6829624E-739.038359
TACGCCG750.001481180138.6665575
GGCCCAC1209.534064E-636.249897145
ATAGGAG800.002033595236.2498935
ATATAGG800.002033595236.2498936
GTGTAGA850.002732379634.131671
TATGGAC850.00273793134.117554
CTACACC1707.781273E-834.117554
TATATTC1501.1292013E-633.8332375
ACACAGT1301.649649E-533.461446
TCCTACA1551.4589586E-632.741842
GTATAAT900.003615545632.2354661
TCCGATC7950.031.9181483
Produced by FastQC (version 0.11.2)