FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319913
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6780.2119326191808398No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6590.20599350448403161No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6080.1900516702978622No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5730.17911119585637345No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5660.17692310096807567No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4930.15410439713297053No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4410.13784997796275864No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4380.136912223010631No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4220.13191086326595042No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT3680.11503127412765346No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG3530.11034249936701541No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3530.11034249936701541No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3450.10784181949467511No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3370.10534113962233482No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA3300.10315304473403705No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA3240.10127753482978184No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA6050.077.9357451
TTCCGAT6450.071.944112
CCGATCT7050.066.849664
CGATCTG4100.056.5901835
CGATCTA1750.049.718525
TCTTCCG3350.043.2872665
CTAGTGC855.3306387E-542.650693
TCCGATC11100.042.4585723
TACACAG1201.9665094E-742.295275
GGGCGAC2100.041.4256257
CCCACGC1001.3860862E-436.264427145
GTCGCCC850.002733872534.13122145
GGGTACC850.00273806834.1205526
GCGACCC3151.8189894E-1229.9185059
GTGCTCT4650.029.640081
GGCGACC3001.8189894E-1128.9979388
TATATAG1000.006070477428.9979367
GTCATAT2104.944577E-727.6343611
TAGACCA1050.00769754327.62144
TCTAGAT1358.0666214E-426.854143
Produced by FastQC (version 0.11.2)