FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346307
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6240.18018694395435264No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5930.17123534898226142No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5850.16892525995720561No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5510.15910738160071844No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5000.1443805640659877No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4380.12647737412180524No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4050.11694825689345004No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3880.11203931771520644No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3700.10684161740883089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT6300.063.301722
CTTCCGA6200.063.180581
CCGATCT6350.062.7942164
CGATCTG3250.053.537555
CGATCTA2100.048.332515
CCCTAGT700.001055021741.4338532
CTGTCCG907.473043E-540.2770969
GTGCTCT4000.038.083851
GCATAAT1406.5641143E-736.2703321
TCCGATC11150.035.7617263
TCTTCCG4150.033.1922075
TATTACT1652.3781176E-630.76152
CCTAGGT950.004720128630.5302092
CTAGACT1000.006069550828.9995084
ACTATAC1000.006069550828.9995083
TAGCGGT1000.006069550828.9995084
TAATAGA1555.4908276E-528.0640414
CCCCCCG1855.80252E-627.4319679
GTCCTAA2156.0753155E-726.9918771
TCCCCCC1907.1387094E-626.7100758
Produced by FastQC (version 0.11.2)