FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324594
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5670.174679753784728No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5540.17067475061153317No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5530.17036667344436435No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5330.1642051301009877No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4950.15249819774857204No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4250.13093279604675379No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4130.1272358700407278No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3980.12261471253319531No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3440.10597854550607837No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3410.10505431400457187No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3300.10166546516571472No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3270.10074123366420822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA7600.078.305551
CCGATCT7550.077.8028644
TTCCGAT7800.076.238942
CCTACCC350.003560448562.159963
CGATCTG4200.058.706635
TCCGATC11600.051.2641033
CGATCTA1700.046.924685
CGATCTC4050.041.1841745
AGGTGCC3250.031.2297427
TAAGGTG3300.030.7660455
GATCTGG3900.029.751786
GTAGTAT1000.006037551429.0303541
ATCTGGG1504.3906555E-528.9990467
TCTCCCC1000.00606947228.9990448
TAGACCA1804.6770965E-628.2022065
CGATCTT3403.6379788E-1227.728225
GTATAAT1050.00766168527.6479551
TCCCCCC1855.8017667E-627.4315268
TACGAGT2405.2346877E-827.1949844
ATACTGT1606.801407E-527.1949836
Produced by FastQC (version 0.11.2)