FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293001
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6140.20955559878635227No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6130.20921430302285657No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5750.19624506401002043No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4900.16723492411288698No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4860.16586974105890423No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4090.13958996726973627No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3840.1310575731823441No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3640.12423165791243032No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3380.11535796806154246No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3280.11194501042658558No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3240.11057982737260283No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3210.10955594008211576No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT3190.10887334855512439No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3180.1085320527916287No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG3000.10238872904870633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA5650.079.566981
CGATCTG3950.071.578575
TTCCGAT6550.069.729212
CCGATCT7150.063.877814
CCTACGG450.00963435848.330833
TCAGGGG951.9168892E-645.78713
GTATATG1002.7252645E-643.505171
TCTGGCC855.333846E-542.6448488
ATCTGCG1053.8161998E-641.4264267
TCCGATC11200.040.7791373
CTCCCCC2005.456968E-1239.8729367
TCCCCCC2109.094947E-1237.9742248
CCCCCCG2151.2732926E-1137.0911039
CCCCAGA850.00273724134.1217041
ACCTGGG1102.4327083E-432.952842
TACGAGT2506.91216E-1131.8983484
GTACATA950.00472309730.5247346
ATACTCC2651.382432E-1030.0927814
ACTCCCC2701.70985E-1029.535516
GTGTAGG1255.1414786E-429.0034481
Produced by FastQC (version 0.11.2)