FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214156
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4290.20032126113674145No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA4010.18724667999028746No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC3950.18444498403033305No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3510.16389921365733393No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT3300.1540932777974934No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3150.14708903789760736No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2930.1368161527111078No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG2930.1368161527111078No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA2900.1354153047311306No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2590.12093987560469939No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC2390.11160088907151795No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2380.11113393974485888No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA2330.10879919311156352No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2310.1078652944582454No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT2260.10553054782495004No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2210.10319580119165468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGG259.3773264E-486.998323
CGATCTG2700.080.5545
CTTCCGA4250.068.233971
CCGATCT4300.065.754554
TTCCGAT4800.060.4155042
GTATAAG901.3188401E-648.33241
ATTATAG450.0096296148.33243
ATTATAC752.5441062E-548.3323973
CCAGCGC951.914028E-645.7885869
TCCCCCC1602.0008883E-1145.3116268
CCCTTAT1002.7244023E-643.499161
CCCCCCG1703.8198777E-1142.6462339
TCTGCGC700.001054880741.427778
TCCGATC7200.040.2773
CCATACT1807.094059E-1140.2772
TATACAT1105.2456253E-639.5446935
CTCATAC951.02630394E-438.1571543
ACTCCCC1901.2732926E-1038.1571546
CTCCCCC1951.6916601E-1037.1787727
CATACTC2151.2732926E-1137.092313
Produced by FastQC (version 0.11.2)