FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868925
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA57750.061.022671
TTCCGAT58700.059.7845882
CCGATCT60300.057.7172854
CGATCTG29900.056.987375
TCCGATC62650.056.1309853
GACTAGT750.001482541638.670582
GATCTGG17700.038.097086
CGATCTC22600.037.8578155
ATCTGGG8300.035.8127567
CGATCTA11450.034.1956025
GTGCTCT34750.031.7172641
TCTGTCC3100.030.4028038
GATCTTA4300.030.3519086
TCTTCCG37750.029.9632955
TGCTCTT37100.029.5110762
TACTAAC1255.1502377E-429.0029372
CGATCTT19500.027.8874385
GATCTGA12000.027.1902526
GCTCTTC40100.027.1224443
ATCTGGC9100.026.2908427
Produced by FastQC (version 0.11.2)