FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565841
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6360.11239906616876472No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA6260.11063178525416151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG18100.054.073635
CCGATCT38150.050.7396054
CTTCCGA38700.050.602861
TTCCGAT40350.048.691832
TCCGATC43650.045.010663
ATCTGAC4150.034.9391447
CGATCTA7600.034.3414965
ATACTAG850.002741221334.1170436
GATCTAG2653.6379788E-1232.829616
TATACGT900.003627224132.2216532
GATCTGA7450.032.1135256
GTATACG1402.7366399E-531.0846141
CGATCTC14700.030.5776925
ATCTGTG2252.8130671E-828.9994897
GATCTGC13400.028.6748686
GTGCTCT26950.027.1822191
CCATACG1100.00966893826.3631692
CGATCTT10200.026.2985535
GATCTGG11000.025.7040926
TCTTCCG28050.025.5877845
Produced by FastQC (version 0.11.2)