FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270141
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8540.31613120555561725No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7520.27837314587567236No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7450.2757819064858722No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6970.2580134078129569No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6390.23654313858318432No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5750.21285180701929732No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4960.18360781962012432No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG4670.172872685005238No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4580.1695410915040664No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG4250.15732524866643716No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4230.1565848945550657No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4130.15288312399820836No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT3900.14436905171743644No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3860.1428883434946935No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3820.14140763527195058No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA3820.14140763527195058No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT3750.13881639588215045No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3720.13770586471509322No Hit
GATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCG3420.1266005530445212No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT3330.12326895954334958No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG3260.12067772015354945No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC3220.1191970119308065No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT3100.1147548872625777No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC2950.10920223142729167No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA2930.1084618773159202No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA2840.10513028381474859No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT2820.10438992970337713No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC2770.10253904442494846No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG2720.10068815914651978No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAT100.007089263144.991497
CTTCCGA5250.084.295561
TTCCGAT5550.081.046252
CCGATCT5550.081.0312354
CGATCTA1200.072.536025
TCACGGG451.18826865E-464.488412
TACTAGC451.18826865E-464.488412
GGGCGAC3950.060.5660637
CCGGGGC606.772212E-660.4466864
CGATCTG3500.058.0288165
CCCTAGT400.006029829454.4120981
GGCGACC4700.052.443738
AGGGGAG701.6876918E-551.8114475
CGATCTC2050.049.5367975
CGGGCCA450.0096267748.33944145
GCGACCC5350.046.072069
TCCGATC10500.044.2124333
TGGGGCG1652.7284841E-1143.9612275
TCTTCCG2350.043.212955
GGGGCGA5400.042.984316
Produced by FastQC (version 0.11.2)