FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503084
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5040.10018207694937624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA33600.056.9867821
CCGATCT33300.056.8241734
TTCCGAT33800.056.6270642
TCCGATC35700.053.61333
CGATCTG15300.049.7547655
CGATCTA5850.044.615265
CGATCTC13750.041.1271635
TAGACTA800.002035681936.24995
GTGCTCT21050.035.489071
TGCTCTT21250.035.4822542
CATAGAC1251.2638646E-534.7999043
GATCTGA5700.034.342016
TCTTCCG21900.034.098085
TCTGTGC2154.8567017E-1033.7208378
CGATCTT10050.032.4625975
GCTCTTC23300.032.3604283
GTACAGG2101.4313628E-831.08371
ATCTATG2202.2595486E-829.6590127
GTATATA2701.70985E-1029.5487041
GATCTCT5200.029.2787656
Produced by FastQC (version 0.11.2)