FastQCFastQC Report
Fri 1 Jun 2018
H2KH2AFXY_n01_p3c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KH2AFXY_n01_p3c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306768
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6350.2069968184426016No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5800.18906796015229752No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5760.18776404318572992No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5450.17765868669483129No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT5350.17439889427841235No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA4230.1378892192145204No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4090.13332550983153393No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3720.12126427789078392No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3710.12093829864914202No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA3420.11148490064152715No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3400.11083294215824335No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3380.11018098367495958No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT3270.10659521201689875No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3270.10659521201689875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA5650.073.1534351
CGATCTG3650.071.506775
CCGATCT6000.067.666594
TTCCGAT6450.064.06972
CCCTAGA553.2129092E-452.735811
AGGGTGC553.2154968E-452.7272156
TTATACG553.2154968E-452.7272154
TTAGCAC657.3286414E-444.6153343
CCATACG657.3286414E-444.6153344
GTCGCGC1303.687237E-739.0384188
TCCTAGG1001.3892108E-436.249962
GGGCAGT800.002034429536.249966
TCCGATC11600.035.6249583
CCTACAC1551.4606376E-632.74193
GATCTGC3550.032.6760186
TCGCGCG1352.13741E-532.2221879
TCCCCCC1601.8715273E-631.7187168
CGTCTTA950.004722267430.526281145
CCCCCCG1703.0027786E-629.8529079
ATTATAC1255.145649E-428.9999683
Produced by FastQC (version 0.11.2)